To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and ...

This map of 2,268 Drosophila melanogaster protein interactions is annotated to show nuclear, cytoplasmic, and membrane and extracellular localization. (from L. Giot et al., Science, 302:1727–36, 2003.

The yeast two-hybrid (Y2H) assay is one of the most widely adopted methods for high-throughput mapping of binary protein–protein interactions. Y2H data sets 1,2,3 have contributed substantially to ...

Fluorescence resonance energy transfer (FRET) is a technique wherein two light-sensitive molecules transfer energy from a donor molecule to an acceptor molecule. The result is emittance of a ...