Vortex fluidic devices (VFDs) used with immobilized metal affinity chromatography (IMAC) have provided an effective, albeit cumbersome, way to separate His-tagged proteins. Researchers recently ...
Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...
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